Proteus
Recombinant Ubiquitin |
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Catalog #U-001
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| Source | Recombinant human Ubiquitin Hydrolase was expressed in E. coli, and purified by IMAC chromatography. |
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| Molecular Weight | The purified, active, human Ubiquitin Hydrolase is a 26kd protein. |
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| Purity | The Ubiquitin Hydrolase is >95% pure by SDS-PAGE and is suitable for crystallization. | |||||||||||
| Activity | Activity was measured by the cleavage of a fluorogenic substrate (Ubiquitin-AMC) from Sigma (Cat # U-2258) and was detected in a microplate format using a Molecular Devices Gemini microplate reader. Specific activity was measured at 25°C and was > 1,000 p moles of substrate hydrolyzed/min/mg protein at pH 7.5. The protein concentration was determined using N-terminal amino acid sequencing. | |||||||||||
| Formulation | Ubiquitin Hydrolase is stored in 100 mM Tris, pH 8.0. |  |
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| Storage | Samples stored frozen at -80oC. Enzyme activity is labile and repeated freeze-thaw cycles should be avoided. | |||||||||||
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