Recombinant Human Renin C-terminal
hexa-His-tag |
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Catalog #R-002
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| Source |
Recombinant human renin with a C-terminal Hexa His tag is generated by tryptic digestion of its His-tagged zymogen expressed in HEK cells.
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| Molecular Weight |
The purified, glycosylated human renin has a molecular weight ~40 kd. |
| Purity |
>95% by SDS-PAGE |
| Activity |
Activity is measured by the cleavage of a fluorescent peptide substrate (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) from Molecular Probes (Cat # R-2931) and activity is detected in a microplate format using a SpectroMax Gemini microplate reader. Specific activity of Lot # 1 was determined to be 100 pmoles of substrate hydrolyzed/min/μg. |
| Formulation |
Renin is shipped at -20oC in 0.1M sodium acetate pH 4.2 at a concentration of 1.0 mg/ml as determined by Bradford protein assay. |
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| Storage |
Samples stored frozen at –20oC are stable for at least six months. Avoid repeated freeze-thaw cycles. |
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| Quantity |
Price |
| 100 µg |
$525/sample |
| 1 - 1.99 mg |
$2625/mg |
| 2 - 9.99 mg |
$1800/mg |
| 10 - 20 mg |
$1100/mg |
| Larger amounts |
Inquire |
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