What is your company's name?
What is your name? (Required)
What is your email? (Required)
What is your phone number?
Service Requested: (Check all appropriate boxes)
Protein Expression Protein Purification Cell Line Development
Can you provide the name of the protein and any other relevant information (truncation, amino acid substitutions, etc?)
How much final product to you require?
Will the quotation be for multiple proteins, e.g. variants? (Multiple proteins could realize cost savings due to efficiencies of scale)
Yes No
How pure does it have to be?
Is endotoxin level important?
What is the expression system you wish to employ?
E. coli Baculovirus (insect cells) Chinese Hamster Ovary (CHO) cells Human Embryonic Kidney (HEK) 293 cells Other mammalian cell expression Yeast Other (please indicate):
What material(s) will you provide as a starting point?
cDNA Plasmid Cells Viral stock None
Do you know the expected level of expression?
How is the protein expressed?
Secreted Soluble product but intracellular Integral membrane protein Insoluble (inclusion body) Unknown
Are purification tags present? If so, do they have to be removed?
Are antibodies to the product available? If so, do you know a source?
Is the presence of protease inhibitors (EDTA, PMSF, etc.) during extraction and/or purification detrimental to the protein of interest?
Does the protein tend to aggregate, or fall out of solution during purification?
Is there a protocol or publication available that describes the work?
Has this protocol been reproduced in your hands?
How do you want the protein to be delivered (frozen, 4°C, lyophilized, concentration, buffer, aliquot size)?
Do you know if the material is stable under the requested delivery conditions?
What is your time frame?
If this is the first time you have requested a quotation from Proteos, how did you hear about us?
All contracted deliverables will be sent to the customer at the completion of the project.
