Proteos
Expression and Production of Proteins—Service and Research
 |
Starting materials either Proteos produced or Customer produced, Chromatography, AKTA, Protein Analytics, Purified protein, Ion Exchange, Ligand Affinity, Immunoaffinity, IMAC, Hydrophobic Interaction, Size Exclusion Chromatography, SDS PAGE, Analytical SEC, N – terminal Sequence, Analytical Reverse Phase HPLC, Carbohydrate Analysis*, Endotoxin Determination*, Mass Spectroscopy Analysis*, *Outsourced
- from your starting material:cell paste or media
- from insect, mammalian, E. coli or yeast culture produced at Proteos
- Can use published purification methods or develop a purification method for your protein
- Recombinant proteins with or without purification tags
- Tags can be removed with TEV, PreScission etc. during the purification process
- Fusion partners can be used to increase expression
- Chromatography using GE Healthcare AKTA systems
- Affinity Chromatography
- Ni- NTA, Talon resins, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, and anti-Flag
- Ion Exchange Chromatography
- Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX, DEAE Sepharose, SP Sepharose, CM Sepharose
- Hydrophobic interaction Chromatography
- Phenyl and (CH2)n Sepharose
- ImmunoAffinity Chromatography
- Protein A and Protein G Sepharose
- Size Exclusion Chromatography
- Analytical to preparative amounts of protein
- The purified protein to gram quantities