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In addition to custom, individualized projects, Proteos' customers have identified a number of proteins that they wanted to have on a continuing basis or in higher quantities. Responding to these requests, Proteos now provides crystallographic grade recombinant human renin. As the only source of recombinant human renin, we are providing highly purified and characterized enzyme to customers all over the world. Additionally, Proteos has extensive expertise in the production of highly pure BACE (beta-site APP cleaving enzyme) from E. coli for crystallography. Proteos offers other proteins that are highly purified and suitable for crystallography.
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Catalog Number: R-001
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| Source: |
* Recombinant human prorenin was expressed in HEK cells, converted
to renin, and the active enzyme was purified using peptide affinity chromatography. |
| Molecular weight: |
* The purified, active, human renin has a molecular weight ~40 kd.
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| Purity: |
* All of the renin is crystallographic grade, (>99% by SDS-PAGE).
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| Activity: |
* Activity was measured by the cleavage of a fluorescent peptide substrate
(Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) from
Molecular Probes (Cat # R-2931) and activity was detected in a microplate format
using a SpectroMax Gemini microplate reader. Activity was measured as ~100 FU/min/ug
protein at the Km. The protein concentration was
determined using amino acid analysis.
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| Formulation: |
* Renin is stored and shipped frozen in 100mM sodium acetate pH
4.2. |
| Storage: |
* Samples stored frozen or lyophilized at -20°C are stable for
at least six months. Avoid repeated freeze-thaw cycles for frozen samples. |
| Pricing: |
| Quantity |
Price |
| 1-1.99 mg |
$2625/mg |
| 2-9.99 mg |
$1800/mg |
| 10-20 mg |
$1100/mg |
| Larger amounts |
Inquire |
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Catalog Number: P-001
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| Source: |
*Recombinant human prorenin was expressed in HEK cells. |
| Molecular weight: |
* The purified human prorenin has a molecular weight ~45 kd.
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 |
| Purity: |
* All of the prorenin is crystallographic grade, (>99% by SDS-PAGE).
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| Activity: |
* Although the prorenin does not contain renin, it does display a low level
(<5%) of renin activity that is intrinsic to the zymogena.
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| Formulation: |
* Prorenin is stored and shipped frozen in 50 mM Tris pH 8.0 |
| Storage: |
* Samples stored frozen at -20°C are stable for at
least six months. Avoid repeated freeze-thaw cycles. |
| a Heinrikson R. L. et al. (1989)
Amer. Jour. Hyperten. 2:367-380. |
| Pricing: |
| Quantity |
Price |
| 100 µg |
$500/sample |
| 1-1.99 mg |
$2500/mg |
| 2-9.99 mg |
$1700/mg |
| 10-20 mg |
$1,000/mg |
| Larger amounts |
Inquire |
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Catalog Number: C-001
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| Source: |
* Recombinant human Caspase 8 was expressed in E. coli, and
purified out of inclusion bodies. |
| Molecular weight: |
* The purified, active, human Caspase 8 is composed of 11 kDa and 19 kDa subunits.
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| Purity: |
* The Caspase 8 is >95% pure (two chains) by SDS-PAGE and is suitable for
crystallization.
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| Activity: |
* Activity was measured by the cleavage of a chromogenic peptide substrate
(Ac-Ile-Glu-Thr-Asp-pNA) from AnaSpec (Cat # 25258-5) and was detected in a
microplate format using a SpectroMax 250 microplate reader. Specific activity
was measured at 25°C and was > 3,000 n moles of Ac-Ile-Glu-Thr-Asp-pNA
hydrolyzed/min/mg protein at pH 7.5. The protein concentration was determined
using N-terminal amino acid sequencing.
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| Formulation: |
* Caspase 8 is stored in 500 mM Tris, 100 mM Hepes, pH 7.5, 4 mM DTT, 10% sucrose,
0.1% CHAPS.
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| Storage: |
* Samples stored frozen at -80°C. Enzyme activity is labile
and repeated freeze-thaw cycles should be avoided. |
| Pricing: |
| Quantity |
Price |
| 1-1.99 mg |
$3675/mg |
| 2-4.99 mg |
$3150/mg |
| Larger amounts |
Inquire |
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Catalog Number: U-001
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| Source: |
* Recombinant human Ubiquitin Hydrolase was expressed in E. coli,
and purified by IMAC chromatography. |
| Molecular weight: |
* The purified, active, human Ubiquitin Hydrolase is a 26kd protein.
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| Purity: |
* The Ubiquitin Hydrolase is >95% pure by SDS-PAGE and is suitable for crystallization.
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| Activity: |
* Activity was measured by the cleavage of a fluorogenic substrate (Ubiquitin-AMC)
from Sigma (Cat # U-2258) and was detected in a microplate format using a Molecular
Devices Gemini microplate reader. Specific activity was measured at 25°C
and was > 1,000 p moles of substrate hydrolyzed/min/mg protein at pH 7.5.
The protein concentration was determined using N-terminal amino acid sequencing.
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| Formulation: |
* Ubiquitin Hydrolase is stored in 100 mM Tris, pH 8.0.
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| Storage: |
* Samples stored frozen at -80oC. Enzyme activity is labile and
repeated freeze-thaw cycles should be avoided. |
| Pricing: |
| Quantity |
Price |
| 1-1.99 mg |
$2,800/mg |
| 2-9.99 mg |
$1,900/mg |
| 10-20 mg |
$1,300/mg |
| Larger amounts |
Inquire |
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