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Proteos is a flexible resource for our customers. We can follow your protocols and
use your cell lines, cDNAs, purification methods, etc. for carrying out a contract,
and thus provide a Service leading to a product that is at a specified state of purity.
Often our clients require input from Proteos in the way of a Research effort to solve
one or more problems along the production train. For example, if scale-up expression
and production is required but the customer has had problems with a bacterial expression
system due to inclusion body formation, they may well avoid the prospect of trying
to refold the protein to an active form. Proteos has special skills in protein refolding,
and may explore application of our proprietary folding method, which is both elegant
and cost-effective. We may also change the cDNA to optimize needed levels of expression.
Protein crystallographers in large pharma, biotech companies, and academic institutions
look to Proteos to provide the especially high purity proteins required for crystallography.
Our track record of producing crystallographic grade protein is well documented (Proteos
Bibliography) and Proteos has provided highly purified protein to customers throughout
the world. Proteos also has a strategic alliance with Active
Site, a protein crystallographic company in San Diego, so customers requiring
both expression and purification, and x-ray structural analysis can contract all of
this work in one shop.
Protein Expression
- E. coli expression of proteins in scales up to 50 liters
and 100's of mg.
- Feasibility studies in refolding of proteins from purified inclusion bodies.
- Optimization of soluble protein expression in either shake flask or in fermentor
growth conditions.
- Yeast expression of proteins
- Insect cell expression in scales up to 50 liters and 100's of mg.
- Baculovirus expression using either Sf9, Sf21 or High five insect cells
- Expression optimization studies
- Growth in small scale in shake flasks or scale-up in Wave bioreactors
- Drosophila expression
- Mammalian cell expression in scales up to 50 liters and 100's of mg. Special
emphasis on rapid selection of stably expressing cell lines.
- Serum free transfection and selection
- Growth in suspension culture in shake flasks or Wave bioreactor technology
- Transient transfection/expression
- Isolation of stably expressing cell lines
- Adaptation of cells to growth in suspension culture and serum free medium
- Molecular Biology:
- Proteos employs standard molecular biology techniques including cloning, PCR
and sequence analysis.
- Gene modification including site directed mutagenesis
Protein Purification
- Metal Affinity Chromatography
- Use of Proteos proprietary technology enabling the purification of metal affinity
tagged proteins from both insect cell and mammalian cell culture supernatants
- Affinity ligand Chromatography including design and synthesis of peptide affinity
ligands
- Size Exclusion Chromatography
- Ion Exchange Chromatography
- Immunoaffinity Chromatography
- Preparation of antibody affinity columns
- Pilot process development of purification methods
- AKTA Chromatography systems as well as proprietary separation technology
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