Expression and Production of Proteins—Service and Research
Proteos can produce your protein in your choice of expression systems
Baculovirus infected insect cells
Sf9, Sf21, High Five* or Drosophila S2 cells
Expression optimization studies
BIIC’S (baculovirus infected insect cell stocks) production for baculovirus storage
Cytoplasmic and secreted proteins
Selenomethionine labelling
Cell paste production
Concentrated culture supernatant production > 100 liter capacities |
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E. coli
Expression optimization – varying time, temperature, induction conditions or media additives.
Stable isotopic labelling
Cell paste production
Inclusion body purification following your refolding paradigm or a referenced method
100 liter capacities in shake flasks
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Mammalian cells
HEK293, CHO (K1, S, DG44) and other cell lines
Stable transfection pools
Stable clonal isolation
Transient transfection
Stable isotope labelling with amino acids (SILAC)
Cytoplasmic and secreted proteins
Cell paste production
Concentrated culture supernatant production
> 100 liter capacities
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Yeast
Saccharomyces cerevisiae, Pichia*, Kluyveromyces*
Cell paste production
Supernatant concentration
> 100 liter capacities in shake flasks
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