Proteus
Recombinant Human Bace |
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Catalog #B-001
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| Source | The catalytic domain of recombinant human BACE with a C-terminal HIS tag was expressed as inclusion bodies in E. coli cells. The protein was refolded and purified using ion exchange and IMAC chromatography. The purified BACE showed activity using the substrate SEISEVY↓VEFRWKK |
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| Purity | Purity, >95% (see SDS-PAGE.) |
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SEC Chromatography: |
1X30 cm Superdex 200 |
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| Formulation | 1.1 mg/ml in 100 mM Sodium Borate, 200 mM Sodium Chloride, pH 8.50 |
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| Reference | A.G. Tomasselli, D.J. Paddock, T.L. Emmons, A.M. Mildner, J.W. Leone, J.M. Lull, J.I. Cialdella, D.B. Prince, H.D. Fischer, R.L. Heinrikson and T.E. Benson, “High Yield Expression of Human BACE Constructs in Eschericia Coli for Refolding, Purification, and High Resolution Diffracting Crystal Forms” Protein & Peptide Letters, Vol. 15, No. 2, 2008 |
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